ISSN:
1617-4623
Keywords:
Drosophila melanogaster
;
tRNA
;
In vitro transcription
;
Polymerase III
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have examined the 5′ flanking region of a Drosophila melanogaster tRNA 4 Val gene for DNA sequences that influence in vitro transcription efficiency in a homologous, cell-free transcription reaction. Comparison of the template efficiencies of a set of deletion mutants indicated both inhibitory and stimulatory sequences in the 5′ flanking region. When sequences between positions-49 and-70, relative to the start of the coding region of the mature tRNA gene, were deleted template activity increased. Deletions to-10 resulted in a 100-fold drop in the rate of transcription directed by the template with the primary effect resulting from deletion of the nucleotides between-38 and-34. The nucleotides between-38 and-34 may be an example of one general type of positive transcription modulator.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00333585
