Electronic Resource
Springer
Molecular genetics and genomics
113 (1971), S. 316-330
ISSN:
1617-4623
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary For efficient transformation with B. subtilis, recipient cells must be grown to the state termed competence. Previous findings indicated that such competent cells contained DNA which exhibited about 5% single-strandedness. In this work, the physico-chemical properties of this DNA are compared to artificially “nicked” DNA. Evidence is presented that breakdown of the host DNA occurs during growth to competence. Inhibition of this breakdown also prevents the formation of partially single-stranded chromosomes within the competent cells. Use of this DNA as donor in transformation studies indicated a deficiency in biological activity within specific genes. Of three models considered, it is concluded that the results are best explained by the occurrence of single-stranded gaps within the chromosomes of competent cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00272332
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