ISSN:
1432-2048
Keywords:
Carbon metabolism
;
Chloroplasts
;
Darkness
;
Glycolytic pathway
;
Oxidative pentose phosphate cycle
;
Spinacia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The conversion of U-labelled [14C]glucose-6-phosphate into other products by a soluble fraction of lysed spinach chloroplasts has been studied. It was found that both an oxidative pentose phosphate cycle and a glycolytic reaction sequence occur in this fraction. The formation of bisphosphates and of triose phosphates was ATP-dependent and occurred mainly via a glycolytic reaction sequence including a phosphofructokinase step. The conversion, of glucose-6-phosphate via the oxidative pentose phosphate cycle stopped with the formation of pentose monophosphates. This was found not to be because of a lack in transaldolase (or transketolase) activity, but because of the high concentration ratios of hexose monophosphate/pentose monophosphate used in our experiments for simulating the conditions in whole chloroplasts in the dark. Some regulatory properties of both the oxidative pentose phosphate cycle and of the glycolytic pathway were studied.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00387270
