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First attempt to characterize 3H-fucose acrosomal label in spermatozoa (1980)

Kopečný, V. ; Sedláková, Eva ; Čechová, Dana ; [et al.]

Springer

Histochemistry and cell biology 68 (1980), S. 67-74

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ISSN: 1432-119X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Acrosomes of rabbit spermatozoa were labelled by tritiated fucose introduced into the cells during spermatogenesis. The labelling was analysed simultaneously by autoradiography and biochemically. In compact intact acrosomes the labelling was confined strictly to the acrosomal region of the sperm head. In swollen and detached acrosomes the autoradiographic grains were associated mostly with the acrosomal cap. Only in some cells a small proportion of radioactivity was detected to be associated with denuded sperm heads. In acrosomal extracts a considerable share of radioactivity coincided with gel filtration fractions showing esterase activity (BAEE-Nalpha-benzoyl-l-arginine ethyl ester splitting), akin to that exhibited by acrosin.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00498502

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