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Cytochemistry of mouse mast cell reaction to polylysine (1980)

Courtoy, R. ; Boniver, J. ; Simar, L. J.

Springer

Histochemistry and cell biology 66 (1980), S. 49-58

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ISSN: 1432-119X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 μg/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00493245

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