ISSN:
1432-0886
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Abstract The response of cells of small primordia ofVicia faba to3H-TdR and colchicine is discussed. The delayed uptake of3H-TdR shown by cells of small primordia appears to be a property of only 50% of the cells; the remaining never become capable of incorporating3H-TdR. Prom the labeled cells and polyploid cells induced by colchicine the shortest cycle time in small primordia is estimated to be 12 hours. Within a period equal to 1 mitotic cycle, 31–35% of all mitoses are tetraploid, following treatment with colchicine. The remainder are diploid and diploid mitoses were seen for up to 30 hours. These observations are indicative of a heterogeneity for mitotic cycle time in populations consisting of up to 1,500 cells. The percentage labeled mitosis curve of diploid cells was changed in primordia treated with colchicine; higher peaks were found. These results show that even small populations of cells, at the beginning of a morphogenetic system, are very heterogeneous for key cell properties.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02327723
