ISSN:
1432-203X
Keywords:
Key wordsSolanum chrysotrichum
;
Cell suspension culture
;
Spirostanol saponins
;
Antimycotic
;
Large-scale culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050296
