ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract We have found that the upstream region of the isocitrate lyase gene (UPR-ICL) from then-alkane-utilizing yeastCandida tropicalis was functional inSaccharomyces cerevisiae as a novel promoter with non-fermentable carbon sources, such as oleic acid, acetate, ethanol, and glycerol/lactate. The expression of two foreign genes coding for β-galactosidase fromEscherichia coli (LacZ) and glutamate decarboxylase from rat brain was carried out under the control of UPR-ICL. Expression ofLacZ was repressed by glucose and enhanced over 300-fold by acetate. When an expression vector pW13 containing multicloning sites between UPR-ICL and the transcriptional terminator of the isocitrate lyase gene (TERM-ICL) was used, the smaller isoform of glutamate decarboxylase (GAD65) was highly produced in a soluble and active form. These results demonstrate that the novel expression system using UPR-ICL and TERM-ICL fromC. tropicalis is useful for the production of heterologous proteins inS. cerevisiae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00178615
