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Enhancement of penicillin acylase activity by cultivating immobilized Kluyvera citrophila (1980)

Morikawa, Yasushi ; Karube, Isao ; Suzuki, Shuichi

Springer

Applied microbiology and biotechnology 10 (1980), S. 23-30

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ISSN: 1432-0614

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary Whole cells of Kluyvera citrophila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%–70% of native cells. When the immobilized cells were continuously cultivated for 40 h in an aerated fermentor containing peptone medium and were treated with alkali in order to remove β-lactamase activity, the immobilized cells produced ampicillin up to 4.4 times faster than noncultivated cells. Ampicillin production was investigated in a column system using these cultivated immobilized whole cells. The cultivated immobilized cells showed excellent performance in continuous ampicillin production.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00504724

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