ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The development of a microassay for proteinase K esterase activity with carboxylic acid esters is reported using novel substrates of the general formula R-C(O)-XR′. Highest rates of hydrolysis have been obtained with the O-phenyl esters CH3(CH2)n = 1 – 2-S-(CH2)n = 1 – 2 C(O)-O-phenyl and their thioester analogs in studies where R, X and R′ have been varied. The phenol release has been measured with 4-aminoantipyrine and potassium ferricyanide to determine the rates of O-phenyl ester hydrolyses. Thioester hydrolyses have been monitored continuously with 5,5′-dithio-bis (2-nitrobenzoic acid).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s0021663540490