ISSN:
1432-072X
Keywords:
Bacterial aggregation
;
Flagella
;
Bacterial motility
;
Bacterial attachment
;
Pseudomonas marina
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pseudomonas marina (ATCC 27 129) rapidly aggregates when suspended in buffered artificial seawater (ASW). Light microscopic observations of stained preparations, showed that flagella-flagella contact was responsible for this phenomenon. Aggregation did not occur if flagella were sheared off, or if motility was inhibited with NaN3. Aggregates were not observed when Mg2+ was omitted from ASW, even though the bacteria remained motile. Other divalent cations, including Ca2+, Mn2+, and Ba2+ could replace Mg2+. However, there is no absolute requirement for divalent cations, since aggregation occurred in ASW containing Cs+ or Li+ instead of Mg2+. P. marina aggregates developed from pH 5.8–8.4, but not below pH 5.8 even though motility continued unimpaired to pH 4.5.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00409246
