ISSN:
1432-072X
Keywords:
Alcohol dehydrogenase
;
Acetaldehyde dehydrogenase
;
Clostridium kluyveri
;
Electron microscopy
;
Polygonal bodies
;
Enzyme complex
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The alcohol-acetaldehyde dehydrogenase complex of Clostridium kluyveri has been separated from contaminating β-hydroxybutyryl-CoA dehydrogenase by repeated precipitation with manganese and ammonium sulfate. Mn++ was required for maximum alcohol dehydrogenase activity. The molecular weight of the enzyme complex was 194,000 as determined by sucrose density gradient centrifugation. The enzyme complex has been shown to contain two types of subunits with molecular weights of 55,000±2,600 and 42,000±1,200, respectively which are arranged in “H”-shaped particles. In solutions with an ionic strength above 25 mM the enzyme complex precipitated in the form of lumps as has been shown with specific ferritin-conjugated antibodies. These lumps are assumed to be aggregated polygonal bodies present in C. kluyveri.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00423073
