ISSN:
1432-2242
Keywords:
Transgenic chicken
;
Avian leukosis virus
;
Avian leukosis resistance
;
Defective ALV
;
Recombinant avian retrovirus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary This report describes the unique biological properties of a transgenic chicken line that contains a defective avian leukosis virus (ALV) proviral insert that we call alv6. Chick embryo fibroblasts (CEF) containing this insert express subgroup A envelope glycoprotein since they yield focus-forming pseudotype virus when co-cultivated with transformed quail cells expressing envelope-defective Bryan high-liter Rous sarcoma virus (RSV). In addition, these cells display high interference to subgroup A RSV but not to subgroup B RSV infection. Chickens containing this insert are highly resistant to pathogenic subgroup A ALV infection, but show little immunological tolerance to subgroup B ALV infection. Thus we have artificially inserted a dominant gene for resistance to avian leukosis infection into the chicken germ line.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00274263
