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Improved rate of callus induction from rice anther culture following microscopic staging of microspores in iron alum-haematoxylin (1987)

Gupta, H. S. ; Borthakur, D. N.

Springer

Theoretical and applied genetics 74 (1987), S. 95-99

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ISSN: 1432-2242

Keywords: Microspore ; Anther culture ; Haploid production ; Oryza sativa L. ssp. indica

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary High frequencies of callusing were obtained in indica rice from the microspores which were staged in acetic acid iron alum-haematoxylin stain prior to culture on G5 medium. Two local varieties, ‘Khonorullo’ and ‘Namyi’, and two advance pre-release cultures, ‘PK 1-1-3’ and ‘PK 12-22’, were used in this investigation. All the cultures exhibited a wide adaptation to varying medium; however, the frequency of callusing was highest (45.5%) in ‘PK 1-1-3’ followed by ‘PK 12-22’ (32.4%) and ‘Khonorullo’ (31.6%). Cold shock (10 °C) for 11 days enhanced the frequency of callusing by 200% in ‘Khonorullo’.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00290090

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