ISSN:
1573-9686
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract Porcine fibrinogen was extracted from blood and dissolved at different concentrations in two types of solvents. Both types had the same ionic strength but differed in pH and osmolarity, and thus had different fibrinogen solubilities. Each type of fibrinogen solution was divided and used to expose two tubes of silicone rubber. One tube of each pair was primed normally, and the other was subjected to our procedure for removing the air nuclei from the surface roughness during the priming procedure. It was found, depending on fibrinogen concentration, pH, and osmolarity of the solvent, that the adsorption to normally primed silicone rubber was as much as 50% greater than on the denucleated material. At conditions corresponding to physiological, the adsorption was approximately 8% greater on normally primed material. If the surface concentration is greater than approximately 0.4 μg/cm2, there is evidence from the isotherm of lateral of multi-layer interaction which may be an indication of denaturing of adsorbed fibrinogen. The increase in adsorption due to the air nuclei indicates that the level of adsorption at each air nucleus on the surface is much above that at which interactions take place between the adsorbed molecules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02364221
