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Plant regeneration of the legume Lens culinaris Medik. (lentil) in vitro (1986)

Williams, David J. ; McHughen, Alan

Springer

Plant cell, tissue and organ culture 7 (1986), S. 149-153

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ISSN: 1573-5044

Keywords: Lens culinaris ; lentil ; regeneration ; organogenesis ; legume

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Until recently, grain legumes in general have proven recalcitrant at de novo regeneration in vitro. By culturing portions of lentil (Lens culinaris) shoot meristems and epicotyls on a medium containing kinetin and gibberellic acid, callus tissue was produced which could be induced to regenerate shoots in relatively large numbers, even after several subcultures. The shoots could be rooted in a mist chamber to yield whole, fertile plants.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00043039

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