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Hypomethylation of repair patches in HeLa cells (1985)

Eremenko, T. ; Palitti, F. ; Morelli, F. ; [et al.]

Springer

Molecular biology reports 10 (1985), S. 177-182

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ISSN: 1573-4978

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract In HeLa cells, under conditions where normal semiconservative synthesis is suppressed by hydroxyurea, the excision repair process after irradiation by UV results in a small amount of incorporation of nucleotides into nonreplicated DNA. By labelling the cytosine moieties of these repair patches, and measuring the ratio between cytosine and 5-methylcytosine, we have found that the level of methylation of cytosine in repair patches five hours after UV-irradiation of the cells is about half of that observed in normal semiconservatively synthesized DNA.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00778526

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