ISSN:
1573-3904
Keywords:
kinase substrate
;
non-protected phosphate
;
phosphate protection
;
phosphorous oxychloride
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new and facile synthesis of tyrosine phosphorylated peptides has been developed. Nα-Fmoc-TyrtBu)-OPfp was treated with TFA, phosphorylated with phosphorous oxychloride and the resulting phosphoric acid dichloride was hydrolysed to give Nα-Fmoc-Tyr(PO3H32)-OPfp 1 in an overall yield of 98%. Compound 1 was used in solid-phase peptide synthesis of phosphopeptides 2, 3 and 4, which are fragments of murine adipocyte lipid binding protein. The advantage of using the Pfp ester was the absence of pyrophosphates and other byproducts.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008839805390