ISSN:
1573-3904
Keywords:
alamethicin
;
circular dichroism
;
ELISA
;
hepatitis C
;
spacer
;
tetraethylene glycol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract New hydrophilic linkers of the formula Fmoc- NHCH2CH2COO(CH2CH2O)4X (X = COCH2CH2COOH Fmoc-Ats (2), X = CONHCH2COOH Fmoc-Atg (4), and X = CONHCH2CH2COOH Fmoc-Ata (5)) have been prepared by heterobifunctional modification of tetraethylene glycol as starting material. These linkers represent a useful tool for solid phase peptide synthesis according to Fmoc/tBu strategy. Two examples are presented to illustrate the applicability of these building blocks: (i) spacing between biotin and a peptide epitope of the hepatitis C virus and evaluation in a biotin- streptavidin ELISA, and (ii) coupling of the new linker to the N- and C-terminus of the peptide antibiotic alamethicin to show eventual influences on the peptide's α-helical conformation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008890613419