Electronic Resource
Palo Alto, Calif.
:
Annual Reviews
Annual Review of Plant Physiology and Plant Molecular Biology
48 (1997), S. 297-326
ISSN:
1040-2519
Source:
Annual Reviews Electronic Back Volume Collection 1932-2001ff
Topics:
Biology
Notes:
Abstract Plant transformation is now a core research tool in plant biology and a practical tool for cultivar improvement. There are verified methods for stable introduction of novel genes into the nuclear genomes of over 120 diverse plant species. This review examines the criteria to verify plant transformation; the biological and practical requirements for transformation systems; the integration of tissue culture, gene transfer, selection, and transgene expression strategies to achieve transformation in recalcitrant species; and other constraints to plant transformation including regulatory environment, public perceptions, intellectual property, and economics. Because the costs of screening populations showing diverse genetic changes can far exceed the costs of transformation, it is important to distinguish absolute and useful transformation efficiencies. The major technical challenge facing plant transformation biology is the development of methods and constructs to produce a high proportion of plants showing predictable transgene expression without collateral genetic damage. This will require answers to a series of biological and technical questions, some of which are defined.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1146/annurev.arplant.48.1.297
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