ISSN:
0899-0042
Keywords:
retention mechanisms
;
separation of enantiomers
;
chiral stationary phases
;
equilibrium isotherms
;
bonding
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The retention mechanisms and the separation of enantiomers on the classes of chiral stationary phases which are made by bonding isolated groups on the surface of an adsorbent are discussed. It is shown that retention on these phases originates from mixed mechanisms and how the individual contributions of these two mechanisms can be separated, by determining and modeling the equilibrium isotherms. A contribution originating from interactions of the isomers with the nonselective sites (type-I) and another one due to interactions with the enantioselective sites (type-II) can be determined and their importance studied as a function of several parameters, e.g., temperature or pH. This approach is illustrated with results obtained with different pairs of enantiomers on bovine serum albumin, 4-methylcellulose tribenzoate, or cellobiohydrolase immobilized on silica. Chirality 10:375-381, 1998. © 1998 Wiley-Liss, Inc.
Additional Material:
1 Tab.
Type of Medium:
Electronic Resource
