ISSN:
0886-1544
Keywords:
septate junction
;
α-actinin
;
myosin II
;
vinculin
;
microfilament bundles
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Cytoskeletal elements associated with the smooth septate junctions linking the midgut columnar cells of Manduca sexta larvae (Insecta, Lepidoptera) were characterized. Myosin subfragment 1 decoration and immunostaining for actin demonstrated that the filaments associated with the septate junctions were constituted of actin. Moreover, using a combination of immunochemical and immunolocalization techniques, evidence is presented that α-actinin, myosin II, and vinculin are localized close to the specialized plasma membranes. The insertion of microfilament bundles into submembranous F-actin/α-actinin/vinculin complexes, previously described in vertebrate junctions of adherens type, appears to be a more general organization, including the insect septate junction here examined. © 1993 Wiley-Liss, Inc.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970260304
