ISSN:
0148-7280
Keywords:
Xenopus
;
oocytes
;
meiosis
;
progesterone
;
MPF
;
monensin
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The carboxylic ionophore monensin inhibits the meiotic maturation of the Xenopus oocyte. When oocytes are exposed to high concentrations of monensin (10 μM), both progesterone and MPF-induced (maturation-promoting factor-induced) maturations are blocked. Lower doses of monensin (1-10 μM) do not inhibit the formation or amplification of MPF activity in the oocyte cytoplasm; however, breakdown of the nuclear envelope does not occur. These observations show that monensin, which is known to abolish intracellular proton gradients, interferes with the mechanism of the breakdown of the nuclear envelope induced by MPF.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120090309
