ISSN:
0148-7280
Keywords:
acrosin
;
proflavin-sepharose
;
anti-acrosin antibodies
;
rabbit
;
fertilization inhibition
;
immunoreproduction
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Rabbit acrosin was purified from detergent extract of epididymal spermatozoa by molecular-sieve Chormatography (Sephadex G-75) and Priflavin-Sepharose affinity chromatography. Affinity Purified rabbit acrosin (Specific activity = 63.7 U Per mg Protein) exhibited one major band (Mr = 36,000) on SDS-polyacrylamide-slab gel electrophoresis. Sheep werweantibodies, Preared against acid-glycerol-stabilized acrosin, were Fractionated sequentitally with 50 and 33% ammonium sulfate. Anti-acrosin immunoglobulins were specific for the acrosome as evidenced by indirect fluorescence lebeling, and they inhibited acrosin's proteolytic activity when essayed for their effect on fertilization invivo, antiacrosin-treated sperm produced the lowest persent fertility (7.1%) followed by preimmune y-globulins(69%), Krebs-ringer hosphate-glucose-serum(96.4%) Analysis of antibody-treated sperm revealed no significiant chang in their motility or agglutination patterns. Antiacrosin antibodies, therefore, can effectivily neutrlize acrosin and inhibit fertilization when placed at the site of sperm-egg interaction in vivo.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mrd.1120080207
