ISSN:
0095-9898
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
A contractile protein was isolated from the cow carotid artery by extracting with a medium containing 0.6 M KCL. The Enzymatic activity of the artery contractile protein resulted in the splitting of the terminal phosphate of ATP.Abbreviations: ATP for adenosine triphosphate; ATP-ase for adenosine-triphosphatase; R.F. for relaxing factor; AM for actomyosin; EDTA for ethylenediamine tetraacetic acid; TCA for tiechoroacetic acid; ηrel for relative viscosity; ηrel ATP for relative viscosity after addition of ATP. The divalent metals Ca and Mg activated the enzyme with Ca showing the more pronounced activation. In addition to the studies on the ATP-ase activity other properties were investigated, such as viscosity, solubility in KCL solutions, ATP-induced synersis and sensitivity to relaxing factor. The properties of the contractile protein were those of actomyosin. The protein resembles uterine actomyosin with respect to its low ATP-ase activity and its viscosity values of Zη and ATP sensitivity.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1030650308
