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Selection for temperature-sensitive mutants of diploid and tetraploid mammalian cells (1974)

McBurney, Michael W. ; Whitmore, Gordon F.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 83 (1974), S. 69-74

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Mammalian cell populations may be enriched for temperature-sensitive (ts) mutants by the tritiated thymidine (3H-TdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the near-diploid Chinese hamster ovary (CHO) cell line and on a line made “tetraploid” by Colcemid treatment. Clones of ts mutants were obtained from the diploid line, but in spite of repeated attempts, no ts mutants were isolated from the tetroploid line. The phenotypes of the new diploid ts mutants recovered were consistent with the particular selection regime employed.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1040830110

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