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Cell proliferation in Xenopus tissues: A comparison of mitotic incidence in vivo and in organ culture (1969)

Simnett, John D. ; Balls, Michael

New York, NY : Wiley-Blackwell

Journal of Morphology 127 (1969), S. 363-372

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ISSN: 0362-2525

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Using the Colcemid technique, the mitotic incidence (MI) was measured in the epidermis, lung, spleen, liver, kidney and ovarian follicular cells of metamorphosed, immature Xenopus laevis laevis. The MI was higher at 25°C than at 20°C, and there was a significant ranking correlation between organs in respect of the MI in different animals. With the exception of the liver and kidney, organ cultures showed good preservation for up to six days in vitro using a medium supplemented with 10% fetal calf serum, and values for MI comparable with or even higher than in vivo were obtained.

Additional Material: 2 Tab.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jmor.1051270307

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