ISSN:
1045-4861
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
This study examined the effects biomaterial adherence had on Staphylococcus epidermidis-46 morphology and sensitivity to the antibiotic cephaloridine. Polymethylmethacrylate (PMMA) discs served as the biomaterial substratum in this study. Suspension cultures of SE-46 at 108 CFU/mL were allowed to adhere to PMMA discs for 2, 6, or 12 h prior to exposure to cephaloridine at 250 μg/mL, which is 500 times greater than the MIC of 0.5 μg/mL. After a 24-h exposure to the antibiotic viable bacteria adherent to the disc were removed and counted. Those data revealed that at shorter adherence times a larger percentage of the adherent organism were erradicated by antibiotic exposure, 83.6%versus 15.6%for 2-h and 12-h adherencetimes, respectively. Although a significant percent of the organisms were killed with short adherence times, the organism still persisted in the face of high concentrations of cephaloridine. Scanning electron micrographs of organism adherent for 2 and 12 h revealed distinct morphological changes in the extracellular matrix indicating increased matrix production with longer adherence. Thus, these data reveal that adherence to biomaterials provides a protected environment for the bacteria and the time an organism is adherent to a biomaterial surface prior to antibiotic exposure can also increase the organism's resistance to antibiotics.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jab.770030205
