ISSN:
0006-3592
Keywords:
chloroperoxidase
;
Caldariomyces fumago
;
indole
;
total turnover number
;
space-time yield
;
membrane reactor
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of 〉860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L · d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 283-288, 1997.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
