ISSN:
0006-3592
Keywords:
Streptomyces coelicolor A3(2)
;
actinorhodin
;
chemostat culture
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Streptomyces coelicolor was grown in variously limited chemostat cultures and the specific rate of extracellular actinorhodin production (qactinorhodin) was measured. The highest qactinorhodin values were observed in glucose- or ammonia-limited cultures, whereas almost no actinorhodin was produced in sulfate-, phosphate-, potassium-, or magnesium-limited cultures. The effect of the dilution rate on actinorhodin production was studied in glucose-limited cultures. It was found that qactinorhodin was highest at D = 0.06h-1, which was well below the maximal D value tested (0.14 h-1). This explains why, in batch cultures, actinorhodin production starts at the onset of the stationary phase. It was also found that the use of nitrilotriacetate instead of citrate as a chelating agent had a negative effect on actinorhodin production. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 577-582, 1997.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
