ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The utilization of mixtures of methanol (C1) and glucose (C6) of different composition by the methylotrophic yeast Hansenula polymorpha was studied in carbon-limited chemostat culture. For all mixtures tested a similar utilization pattern was observed: At low dilution rates both carbon sources were utilized simultaneously, but at high dilution rates the cells used glucose only and the unutilized methanol accumulated in the culture medium. When grown with C1 only, the cells exhibited a critical dilution rate Dc(C1) of 0.19 h-1, but when C1-C6 mixtures were used as the carbon and energy substrate, the yeast was able to completely utilize C1 at dilution rates considerably higher than Dc(C1). The dilution rate at which the transition from C1-C6 growth to C6 growth occurred (Dt) was strictly dependent on the composition of the C1-C6 mixture in the feed, and Dt increased with decreasing proportions of C1 in the mixture. During mixed substrate growth the formation of biomass from the two substrates was additive. The results reported indicate that the utilization of C1-C6 mixtures and hence Dt in H. polymorpha are subject to two different regulatory regimes. When the cells were growing with C1-C6 mixtures containing more than 60% C1, the transition form C1-C6 to C6 growth was most probably influenced by the maximum C1 oxidizing capacity of the cells, whereas for growth with mixtures containing less than 40% C1, a growth rate of 0.28-0.30 h-1 seemed to be the limiting barrier for the simultaneous utilization of the components of the binary carbon and energy substrate mixture.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260281118
