ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A simple procedure for the entrapment of enzymes in nylon-reinforced cellulose triacetate fibers and the fabrication of reusable enzyme brushes using such fiber bristles for the analytical determination of unknown samples have been described. As an example, enzyme brushes of lactate dehydrogenase were fabricated and were repeatedly employed for the determination of pyruvic acid in clinical samples in place of soluble enzymes. The above brush method compared well with that of wet enzymic analysis of pyruvate in clinical samples in accuracy, sensitivity, and reproducibility. The device also proved to be easy to handle, use, and reprocess for repeated analyses.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260281104
