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Purification and properties of glucose isomerase of Actinoplanes missouriensis (1980)

Gong, Cheng-Shung ; Chen, Li Fu ; Tsao, George T.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 22 (1980), S. 833-845

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Actinoplanes missouriensis produces an intracellular soluble glucose Isomerase. The soluble enzyme can be purified by a DEAE-cellulose beads columm with a onestep salt elution. The purified enyzme exhibited a molecular weight of approximately 80,000 daltons, being composed of two identical subunits of about 42,000 daltons each. The Km for glucose is 1.33M, the Km for frucotse is 1.67M. The enzyme has an optimal pH of 7.0. The presence of the cobalt ion is not required to produce optimal activity of the enzyme if the proper amount of magnesium is present.

Additional Material: 9 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260220408

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