ISSN:
0006-3592
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Raffinose was converted enzymatically in a hollow-fiber reactor to melibiose, sucrose, galactose, glucose and fructose. The enzymes were a crude extract of α-galactosidase and invertase produced by Aspergillus awamori NRRL 4869 on a solid substrate, Wheat bran. With a concentration of raffinose, C0, entering the reactor at a flow rate Q, and with C being the concentration of raffinose exiting the reactor, the conversion (C/C0), was studied as a function of Q at two level of C0. The data could be fairly well fitted using the analysis of Waterland et al. even though a mixed crude enzyme system was being investigated. It was found empirically that In (C/C0) was linear in Q-1, with the absolute value of the slope decreasing with increasing C0. The linearity of such plots were predicted by Lewis and Middleman from Waterland et al. for a single enzyme system obeying first-order kinetics, the slope being independent of C0. Although the assumptions involved in this approximate analytical solution are not valid, the observed linearity of the In (C/C0) vs. Q-1 plots is excellent and should prove useful in reactor design considerations.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260220306
