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Effect of pH on the growth cycle of HeLa cells in batch suspension culture without oxygen control (1971)

Barton, M. E.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 13 (1971), S. 471-492

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: HeLa cells have been cultured at different controlled pH levels in batch suspension culture with open gas phase. Continuous monitoring of the dissolved oxygen tension throughout the culture cycle reveals a characteristic pattern of variation which correlates with various aspects of growth and carbohydrate metabolism. The effect of pH, size, and physiological state of the inoculum on this pattern have been determined, and the results are discussed in terms of a theoretical respiratory rate equation.

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260130403

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