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Continuous chemical sterilization of microbiological media with β-propiolactone (1962)

Toplin, Irving

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 4 (1962), S. 331-340

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: A laboratory bench set-up for the continuous chemical sterilization of microbiological media using β-propiolactone was developed. The apparatus permits the sterilization of up to 4 liters/hr. of liquid media. The parameters of hydrolyzing time and temperature necessary to reduce the β-propiolactone to nontoxic levels were determined using a E. coli-nutrient broth system. The sterilization technique was applied successfully to a variety of media, and its potential usefulness on an industrial scale is discussed.

Additional Material: 4 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bit.260040309

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