ISSN:
0263-6484
Keywords:
Chromatin
;
phospholipid synthesis
;
rat hepatocytes
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Nuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P] O42- and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were (a) the initial specific activity of the chromatin PL was half that of both nuclear and microsomal PL at 1 h; (b) the first peak of labelling occurred at 6 h in both nuclear and microsomal PL, but was 3 h later (9h) in the chromatin PL; and (c) a second peak of labelling occurred in the chromatin and microsomal PL, but not in those of the nuclei.On fractionation of the PL, the major and most metabolically active components were phosphatidylcholine + phosphatidylethanolamine, whilst sphingomyelin accounted for only about 8 per cent of the total PL. The chromatin and microsomal fractions were somewhat similar in their labelling patterns though with a delayed peaking of activity in the chromatin. This is indicative of a synthesis and transport of PL from the microsomes to the chromatin.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cbf.290040409
