ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Electrofocusing on diagonal immobilized pH gradients was designed to combine high field strength with a flat pH gradient over a wide pH range, circumventing the need for extremely high voltage in long, conventionally oriented focusing gels. The technique was found to be applicable at 500 V cm-1 to gels of 35 cm length of a flat pH gradient with a predicted pH range of 3.8 to 9.8, allowing for a focusing time of 2 h. Note that the equivalent flat pH gradient gel of 25 cm length at the same field strength would require application of 12 500 V. However, preliminary results indicate that the theoretically expected improvement in resolution due to high field strength does not materialize. This may be due to a distorted electric field caused by non-uniform conductance features inherent in the method and by the passage of the current through the isoelectric protein zones.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150070402