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Computer analysis of two-dimensional gels by a general image processing system (1986)

Tyson, John J. ; Haralick, Robert H.

Weinheim : Wiley-Blackwell

Electrophoresis 7 (1986), S. 107-113

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ISSN: 0173-0835

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: In order to survey changes in accumulation of several hundred proteins during the naturally synchronous nuclear division cycle of Physarum polycephalum, we have developed methods for analyzing two-dimensional (2-D) gel electrophoretograms using the general image processing system developed by the Spatial Data Analysis Laboratory at Virginia Tech. In this paper we describe fast and accurate methods for removing non-homogeneous background intensity from a 2-D gel image, for resolving overlapping protein spots, and for estimating the total integrated intensity in a protein spot by Gaussian modeling.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/elps.1150070302

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