ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Enolsilylation of prostaglandins E1 and E2 as well as their 11-desoxyanalogues and the corresponding methyl esters by a mixture of N,O-bis-(trimethylsilyl)acetamide-piperidine (1:1) results in trimethylsilyl ether derivatives of Δ8(9)-enol forms contaminated by c.10% Δ9(10)-enol derivatives. Isomeric PGE2 9-enol-trimethylsilyl ether derivatives are separable by capillary gas-liquid chromatography and their mass spectra show different fragmentations of the side chains diagnostic for the above structures. Therefore, the use of 9-enol-trimethylsilyl ethers for prostaglandins E analysis in biological fluids could lead to formation of the two peaks in the gas chromatogram and moreover does not discriminate between common and 8-iso-prostaglandins E.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200100703
