ISSN:
0306-042X
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Leucine enkephalin is measured in canine spinal cord tissue in a structurally unambiguous manner. A rapid tissue procurement procedure minimizes enkephalin metabolism. High-performance liquid chromatography purification of brain neuropeptides is followed by field desorption mass spectrometric measurement of leucine enkephalin in spinal cord tissue extracts. Quantification is performed at the 70 ng (126 pmol) g-1 of wet weight tissue, or 70 parts per billion level. The higher homolog of leucine enkephalin, 2ala-leucine enkephalin, is utilized as internal standard. Straight-line statistics are obtained for a series of samples to which a peptide standard is added.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200100604
