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The combined use of enzymatic hydrolysis and fast atom bombardment mass spectrometry for peptide sequencing (1983)

Self, R. ; Parente, A.

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 10 (1983), S. 78-82

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ISSN: 0306-042X

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub-peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained from spectra recorded on 1-5 nmol samples after digestion at 37°C for times ranging from 0.1-4.0 h. The qualitative results of attack by two exopeptidases on three oligopeptides are discussed.

Additional Material: 3 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bms.1200100206

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