ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
This paper reports the application of matrix-assisted UV-laser induced desorption (LID) mass spectrometry for the analysis of some extracellular multi-domain cellulolytic enzymes, in the molecular weight range 5000-90 000 u, produced by the fungi Phanerochaete chrysosporium and Trichoderma reesei. A high sensitivity (1-10pmol of protein), has been attained in obtaining the mass spectra of these highly glycosylated enzymes and some of their peptides, with carbohydrate content in the 10-40% range. The desorption of samples containing the enzymes mixed with a protein having an accurately known molecular weight - an internal calibrant - is also demonstrated. Spectra from complete enzymes and their functional domains are presented to explore the possibilities of employing LID for obtaining structural information.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290040806
