ISSN:
0935-6304
Keywords:
Micellar electrokinetic capillary chromatography
;
temperature programming
;
applied voltage programming
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
---Temperature and voltage programming modes were utilized to optimize selectivity and increase the eluting rate of strongly retained compounds in micellar electrokinetic chromatography. Separations obtained by applying temperature, voltage, and a simultaneous combination of temperature and voltage gradient in micellar electrokinetic capillary chromatography were compared with separations performed under isothermal and constant voltage conditions. A complete separation of all the constituents of the test mixture was only achieved in the temperature programming run and in a combination of temperature and voltage programming modes. Simultaneous variations of column temperature and applied voltage during a separation run, yielded a 30% reduction in the total analysis time when compared to a temperature gradient alone. Temperature programming and voltage programming modes may be the gradient methods of choice because of the considerable technical difficulties involved in performing linear solvent gradient elution in micellar electrokinetic chromatography.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
