ISSN:
0935-6304
Keywords:
Supercritical fluid chromatography, SFC
;
Three-dimensional diagrams
;
Capacity ratio
;
Resolution
;
Temperature dependence
;
Pressure dependence
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
For chromatography with supercritical fluids (SFC), the dependence of the capacity ratios k′ and of the mean resolution Rm on temperature and pressure is presented as a three-dimensional diagram. A sufficient number of test chromatograms were run for the diagram to lead to an isocratic network in form of a curved surface. The isocratic network possesses a characteristic shape and contains all information about the temperature and pressure dependence of k′ and Rm for a given volume flow rate and chromatographic system. The specific system studied comprised pentane as the mobile phase, unmodified silica as the stationary phase, and a set of four polycyclic hydrocarbons as the test mixture. The isocratic net of this system allows interpolation of k′ and Rm for any temperature and pressure. Together with similar experimental data from other systems, this allows qualitative forecasts about the isocratic nets of other systems.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240091005
