ISSN:
0935-6304
Keywords:
Liquid chromatography
;
Reverse phase HPLC
;
Absorbance ratio
;
Spectro-chromatograms
;
UV-VIS detector
;
Multichannel diode array
;
Purity control by HPLC
;
Screening for metabolites in dog bile
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
This article describes the use of a multichannel UV-VIS detector coupled on-line to an HPLC instrument. Simultaneous measurements of the absorption of a compound over a full range of wavelengths (200-800 nm) makes this almost universal detector a useful tool in screening for unknown compounds. The procedures used to compute so-called spectro-chromatograms and to draw pseudo-three-dimensional plots are discussed. The experimental examples given include an application to screening for drug metabolites in biological fluids.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240050405
