Quantitation (theory, practice)
Effect of substance distribution within layer on quantitative data
Secondary chromatography effects responsible for most of error sources
Analytical Chemistry and Spectroscopy
Wiley InterScience Backfile Collection 1832-2000
Chemistry and Pharmacology
Changes of fluorescence intensity at the far side (transmission) and near side (remission) in cases where a spot of a fluorogen lies in different sublayers are calculated using a multilayer model. Examples shown are: the relative intensity of fluorescence in the layer as a function of the absorption coefficient of a sorbent at the excitation wavelength; the relative intensity of fluorescence at the far and near sides of a TLC plate; the influence of using too broad a monochromatic filter at the emission side on the results of fluorescence measurements. Advantages of near-side scanning of fluorescence compared to far-side scanning are discussed. The most important source of errors in fluorodensitometric measurements is the effect of secondary chromatography.
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