ISSN:
0935-6304
Keywords:
Thin-layer chromatography
;
Quantitation (theory, practice)
;
Effect of substance distribution within layer on quantitative data
;
Secondary chromatography effects responsible for most of error sources
;
Fluorodensitometry
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Changes of fluorescence intensity at the far side (transmission) and near side (remission) in cases where a spot of a fluorogen lies in different sublayers are calculated using a multilayer model. Examples shown are: the relative intensity of fluorescence in the layer as a function of the absorption coefficient of a sorbent at the excitation wavelength; the relative intensity of fluorescence at the far and near sides of a TLC plate; the influence of using too broad a monochromatic filter at the emission side on the results of fluorescence measurements. Advantages of near-side scanning of fluorescence compared to far-side scanning are discussed. The most important source of errors in fluorodensitometric measurements is the effect of secondary chromatography.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240030408
