Publication Date:
2022-05-25
Description:
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Archives of Biochemistry and Biophysics 478 (2008): 7-17, doi:10.1016/j.abb.2008.07.007.
Description:
Glutathione S-transferases (GST) were characterized from the digestive gland of
Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these
detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral
diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a
proteomic approach involving affinity chromatography, HPLC and nanospray liquid
chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were
identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative
theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma
GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from
peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and
revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights
into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope
with their chemically defended prey.
Description:
Support was provided by the WHOI-Cole Ocean Ventures Fund (KEW), the WHOI Ocean Life
Institute (KEW and MEH), a grant from Walter A. and Hope Noyes Smith (MEH), the National
Science Foundation Graduate Research Fellowship (KEW), and by the National Institutes of
Health (P42-ES007381 and R01-ES015912 to JVG).
Keywords:
Cyphoma gibbosum
;
Glutathione S-transferase
;
Gorgonian
;
Natural product
;
Chemical ecology
;
Allelochemical
;
Proteomic
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
Format:
application/pdf
