ISSN:
1432-0789
Schlagwort(e):
Key wordsBradyrhizobium japonicum
;
Bradyrhizobium elkanii
;
Repeated sequence RSα
;
Polymerase chain reaction detection
;
DNA hybridization
;
Soil inoculant
;
Nodulation tests
;
N2 fixation
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Geologie und Paläontologie
,
Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
Notizen:
Abstract The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00335812
