Abstract
The genome of Brahyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Brahyrhizobium strains capable of nodulating soybean was also demonstrated.
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Hartmann, A., Gomez, M., Giraud, J.J. et al. Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii . Biol Fertil Soils 23, 15–19 (1996). https://doi.org/10.1007/BF00335812
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DOI: https://doi.org/10.1007/BF00335812